Journal: Biomicrofluidics

Article Title: Monitoring bacterial biofilms with a microfluidic flow chip designed for imaging with white-light interferometry

doi: 10.1063/1.4985773

Figure Lengend Snippet: Diagram of the cross-section of the flow cell when air is inserted into the viewing chamber for imaging. Light from the microscope passes through the top cover glass and through the biofilm. Light reflected from the biofilm/air bubble interface returns to the microscope and creates an interference pattern on the camera when it combines with light reflected from a reference arm in the objective lens. A vertical scanning range is selected to avoid light reflected from the top surface of the coverglass and any lower interfaces. This configuration allows for non-destructive imaging of a biofilm attached to the top cover glass. Biofilm on the opposing surface can be imaged by inverting the flow cell.

Article Snippet: 17 The viewing windows were 25 mm by 50 mm No. 2 microscope cover glass that is 190 μ m to 250 μ m thick (Ted Pella).

Techniques: Imaging, Microscopy

Journal: Molecules

Article Title: In Vitro and Ex Vivo Antifungal Activities of Metconazole against the Rice Blast Fungus Pyricularia oryzae

doi: 10.3390/molecules29061353

Figure Lengend Snippet: Microscopic observation of the antifungal effects of metconazole on the P. oryzae strain P131 ( a ) Bright field (Bar: 200 μm) and ( b ) SEM images (Bar: 2 μm) of hyphal structures in P. oryzae strain P131 on CM plates. ( c ) FDA staining of P. oryzae conidia incubated on hydrophobic glasses at 2 hpi (Bar: 100 μm). MET: methanol as solvent control.

Article Snippet: A conidial suspension of 1 × 10 5 conidia/mL was supplemented with metconazole at a series of concentrations (10, 25 and 50 μg/mL), and a 30 μL droplet of the mixture was incubated on hydrophobic cover glass (48366-067, VWR Corporation, Radnor, PA, USA) at 28 °C under darkness for 2, 8 and 24 h, respectively.

Techniques: Staining, Incubation, Solvent